ABSTRACT
Objective:To explore the efficacy of gold micro needle radiofrequency combined with syringe needle subsicion therapy in the treatment of facial acne scars.Methods:A total of 58 patients with facial depression acne scars admitted to our hospital from May 2018 to May 2020 were selected as subjects. There were 28 cases in the control group and the rest was the experimental group. The control group was treated with gold micro needle radiofrequency, and the experimental group was given a small syringe needle on the basis of the control group. After the treatment, the treatment effect and delayed construction period were observed.Results:According to the results of two independent samples Wilcoxon rank sum test, the treatment results of the two groups were different ( Z=2.742 and P=0.006). The effect of experimental group (mean rank 34.74) was better than that of control group (mean rank 23.88). According to =0.05 level, there was no statistically significant difference between the two groups in terms of missed work time after three treatments. There was no significantly difference between the two groups. Conclusions:Gold micro needle radiofrequency combined with syringe needle subsicion therapy for the treatment of facial acne scar has a satisfactory effect, and it is worthy of clinical promotion.
ABSTRACT
In women with preeclampsia (PE), endothelial cell (EC) dysfunction can lead to altered secretion of paracrine factors that induce peripheral vasoconstriction and proteinuria. This study examined the hypothesis that PE sera may directly or indirectly, through human umbilical vein ECs (HUVECs), stimulate phospholipase C-gamma1-1,4,5-trisphosphate (PLC-gamma1-IP3) signaling, thereby increasing protein kinase C-alpha (PKC-alpha) activity, collagen I expression and intracellular Ca2+ concentrations ([Ca2+]i) in human umbilical artery smooth muscle cells (HUASMCs). HUASMCs and HUVECs were cocultured with normal or PE sera before PLC-gamma1 silencing. Increased PLC-gamma1 and IP3 receptor (IP3R) phosphorylation was observed in cocultured HUASMCs stimulated with PE sera (P<0.05). In addition, PE serum significantly increased HUASMC viability and reduced their apoptosis (P<0.05); these effects were abrogated with PLC-gamma1 silencing. Compared with normal sera, PE sera increased [Ca2+]i in cocultured HUASMCs (P<0.05), which was inhibited by PLC-gamma1 and IP3R silencing. Finally, PE sera-induced PKC-alpha activity and collagen I expression was inhibited by PLC-gamma1 small interfering RNA (siRNA) (P<0.05). These results suggest that vasoactive substances in the PE serum may induce deposition in the extracellular matrix through the activation of PLC-gamma1, which may in turn result in thickening and hardening of the placental vascular wall, placental blood supply shortage, fetal hypoxia-ischemia and intrauterine growth retardation or intrauterine fetal death. PE sera increased [Ca2+]i and induced PKC-alpha activation and collagen I expression in cocultured HUASMCs via the PLC-gamma1 pathway.
Subject(s)
Adult , Female , Humans , Pregnancy , Young Adult , Apoptosis , Calcium/metabolism , Cell Line , Cell Survival , Cells, Cultured , Coculture Techniques , Collagen Type I/analysis , Human Umbilical Vein Endothelial Cells , Muscle, Smooth, Vascular/cytology , Phospholipase C gamma/genetics , Pre-Eclampsia/blood , Protein Kinase C-alpha/metabolism , RNA Interference , Signal TransductionABSTRACT
Objective:To evaluate the application of puncturing the umbilical cord under the ultrasound guidance in prenatal diagnosis,clinical high risk factors of fetal chromosomal abnormalities were discussed.Methods:283 pregnant women who had the percutaneous umbilical cord blood sampling were recruited and the karyotyping was done.The detection rate of chromosomal abnormalities and correlate factors in different subgroups of prenatal diagnosis were analyzed.Results:The success rate of puncture was 100%.Major complications were bleeing at the puncture site(33 cases,11.67%)and fetal heart brachycadia(5 cases,1.77%).25 chromosomal abnormalities were found and the detection rate was 8.83%.There was significant correlation between the couple's chromosomal abnormalities and the fetal's(r=22.348,P=0.000).Conclusions:The ultrasound guided percutaneous umbilical cord blood sampling proves to be valid in the prenatal diagnosis,especially when couples have chromosomal abnormalities.
ABSTRACT
Objective To investigate the incidence of vulvovaginal candidiasis(VVC)in the third trimester,and the effect on the of perinatal outcome.Methods Vaginal discharge samples of 300 gravida were examined and cultured for fungus.According to the clinical feature,the patients were divided into slight VVC group and heavy VVC group.The termination of pregnancy and perinatal outcome were recorded 42 days after delivery.Results The incidence of VVC was 34%.There was significantly higher prevalence of premature rupture of membrane(PROM),afterbirth adherence,premature labor,break of perinaeum when intrapartum,disruption of wound after intrapartum and infection of newborn in the group of heavy VVC,with significant difference as compared with non-VVC group (P＜0.01)but with no difference in puerperal infection(P＞0.05).Conclusion Heavy VVC is disadvantageousto the mothers and their babies in gravida,and the therapy of VVC in pregnant women should be conducted actively before and after delivery.